LOCUS       sequence-103817-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive repeat-only E. coli CRISPR array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Luo ML, Mullis AS, Leenay RT, Beisel CL
  TITLE     Repurposing endogenous type I CRISPR-Cas systems for programmable 
            gene repression.
  JOURNAL   Nucleic Acids Res. 2014 Oct 17. pii: gku971.
  PUBMED    25326321
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Oct 17. pii: gku971."
COMMENT     Sequence Label: pcrRNA.con
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     178..195
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     promoter        complement(331..365)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     primer_bind     complement(460..479)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(617..639)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     780..799
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tcgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc atggggagac
       61 cccacactac catcggcgct acggcgtttc acttctgagt tcggcatggg gtcaggtggg
      121 accaccgcgc tactgccgcc aggcaaattc tgttttatca gaccgcttct gcgttctgat
      181 ttaatctgta tcaggctgaa aatcttctct catccgccaa aacagccaag cttaaaaaaa
      241 accccgccct gtcaggggcg gggttttttt ttcggtttat ccccgctggc gcggggaact
      301 cgaggtggta ccgagctcga attcgctagc gctagcatta tacctaggac tgagctagct
      361 gtcaaatgca tcgatgataa gctgtcaaac atgagcagat cctctacgcc ggacgcatcg
      421 tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg
      481 atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg
      541 tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttctgcctc gcgcgtttcg
      601 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
      661 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
      721 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
      781 ggcatcagag cagattgtac tgagagtgca ccatatgcgg
//
