LOCUS       sequence-103818-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PhoRadAC14 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSABAD14-167
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..28)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             264..286
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(313..597)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(371..390)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 gccaagctta atgatgatga tgatgatgga attccgttac ggtgtaggtt ttggtagcgt
       61 cgtcgtaggt ccattcaccg tcaacaccgt tgtcgttagc gtactgtttg aaaacttttt
      121 ccgcggtagc agcgtcaaca gcttcggtgg tggtttcacc tttcagggtt ttaccgttca
      181 ggataagttt gtactgggta cctagctgag tattatggag aacaagtcca ttgggagcta
      241 tgaagttgtg ggtcatatgt atatctcctt cttaaagtta aacaaaatta tttctagccc
      301 aaaaaaacgg gtatggagaa acagtagaga gttgcgataa aaagcgtcag gtaggatccg
      361 ctaatcttat ggataaaaat gctatggcat agcaaagtgt gacgccgtgc aaataatcaa
      421 tgtggacttt tctgccgtga ttatagacac ttttgttacg cgtttttgtc atggctttgg
      481 tcccgctttg ttacagaatg cttttaataa gcggggttac cggtttggtt agcgagaaga
      541 gccagtaaaa gacgcagtga cggcaatgtc tgatgcaata tggacaattg gtttcttctc
      601 tgaatggcgg gagtatgaaa agtatggctg aagcgcaaaa tgatcccctg ctgccgggat
      661 actcgtttaa tgcccatctg gtggcgggtt taacgccgat tgaggccaac ggttatctcg
      721 atttttttat cgaccgaccg ctgggaatga aaggttatat tctcaatctc accattcgcg
      781 gtcagggggt ggtgaaaaat c
//
