LOCUS       sequence-103821-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NpuDnaEdN11 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSABAD14-HY43
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             65..87
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             659..676
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(716..733)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 tggacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttt gggctagaaa
       61 taattttgtt taactttaag aaggagatat acatatggaa tatggattat taccgattgg
      121 taaaattgta gaaaagcgca tcgaatgtac tgtttatagc gttgataata atggaaatat
      181 ttatacacaa cctgtagcac aatggcacga tcgcggagaa caagaggtgt ttgagtattg
      241 tttggaagat ggttcattga ttcgggcaac aaaagaccat aagtttatga ctgttgatgg
      301 tcaaatgttg ccaattgatg aaatatttga acgtgaattg gatttgatgc gggttgataa
      361 tttgccgaat atcaaaatag ccacacgtaa atatttaggc aaacaaaatg tctatgacat
      421 tggagttgag cgcgaccata attttgcact caaaaatggc ttcatagctt ctaattgttt
      481 caatggtacc cagtacaaac ttatcctgaa cggtaaaacc ctgaaaggtg aaaccaccac
      541 cgaagctgtt gacgctgcta ccgcggaaaa agttttcaaa cagtacgcta acgacaacgg
      601 tgttgacggt gaatggacct acgacgacgc taccaaaacc tacaccgtaa cggaattcca
      661 tcatcatcat catcattaag cttggctgtt ttggcggatg agagaagatt ttcagcctga
      721 tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta
      781 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg
      841 gtagtgtggg gtctccccat gcgag
//
