LOCUS       sequence-103824-        1112 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of N terminal cyclin A and cks1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Di Fiore B, Pines J
  TITLE     How cyclin A destruction escapes the spindle assembly checkpoint.
  JOURNAL   J Cell Biol. 2010 Aug 23;190(4):501-9.
  PUBMED    20733051
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2010-08-23"; volume: "190(4)"; pages: "501-9"
COMMENT     Sequence Label: pT7 cyclin A1-164 venus cks1 (2196)
FEATURES             Location/Qualifiers
     source          1..1112
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(596..615)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(597..615)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(660..863)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(689..709)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agctcctcgc ccttgctcac catggtggcg accggtggat cccgttctaa tacaattgac
       61 atgtccatag tatgtggtga ctcaaaacta ccatccattg gataatcaag agggaccaat
      121 ggttttctgg gtccaggtaa actaatggct gaattaaaag ccagggcatc ttcacgctct
      181 attttttgag attcagctgg cttcttctga gcttcttttt ctgcttcatc cacatgaatg
      241 gtgaacgcag gctgtttact gtttgctttc caaggaggaa cggtgacatg ctcatcattt
      301 acaggaagat ccttaagggg tgcaacccgt ctcgtcttcg gcctctgctg ctgcgctaga
      361 ccccgcgggt tcccggactt cagtaccgcc agcgcggccc gggtccgcgg ttgttggacg
      421 ggcgctgcct tttccgggtt gatattctcc tggtcctctt ggagcgccgt ctgctgcaat
      481 gctagcagcg ccgagcccgc ctcgcgggtc gcaggccccg gcgcagagtt gcccaccatg
      541 aattcgaagc ttgagctcga gatctgagtc cggtagcgct agcaagcttg ggtctcccta
      601 tagtgagtcg tattaatttc gataagccag taagcagtgg gttctctagt tagccagaga
      661 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
      721 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
      781 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
      841 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc
      901 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
      961 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
     1021 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
     1081 atgggaacat acgtcattat tgacgtcaat gg
//
