LOCUS       sequence-103825-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of N terminal cyclin A and cks1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Di Fiore B, Pines J
  TITLE     How cyclin A destruction escapes the spindle assembly checkpoint.
  JOURNAL   J Cell Biol. 2010 Aug 23;190(4):501-9.
  PUBMED    20733051
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2010-08-23"; volume: "190(4)"; pages: "501-9"
COMMENT     Sequence Label: pT7 cyclin A1-164 venus cks1 (2196)
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      550..559
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(556..558,559..561,562..1020)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     complement(601..622)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(862..881)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgctaccgga ctcagatctc gagctcaagc ttcgaattca tggtgggcaa ctctgcgccg
       61 gggcctgcga cccgcgaggc gggctcggcg ctgctagcat tgcagcagac ggcgctccaa
      121 gaggaccagg agaatatcaa cccggaaaag gcagcgcccg tccaacaacc gcggacccgg
      181 gccgcgctgg cggtactgaa gtccgggaac ccgcggggtc tagcgcagca gcagaggccg
      241 aagacgagac gggttgcacc ccttaaggat cttcctgtaa atgatgagca tgtcaccgtt
      301 cctccttgga aagcaaacag taaacagcct gcgttcacca ttcatgtgga tgaagcagaa
      361 aaagaagctc agaagaagcc agctgaatct caaaaaatag agcgtgaaga tgccctggct
      421 tttaattcag ccattagttt acctggaccc agaaaaccat tggtccctct tgattatcca
      481 atggatggta gttttgagtc accacatact atggacatgt caattgtatt agaacgggat
      541 ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      601 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      661 ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc
      721 gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac
      781 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      841 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      901 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      961 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc
     1021 gacaagcaga agaacggcat caaggccaac tttc
//
