LOCUS       sequence-103826-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NpuDnaEdN11 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSABAD14-HY43
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..23)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             595..617
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(644..809)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(702..721)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 gcttaatgat gatgatgatg atggaattcc gttacggtgt aggttttggt agcgtcgtcg
       61 taggtccatt caccgtcaac accgttgtcg ttagcgtact gtttgaaaac tttttccgcg
      121 gtagcagcgt caacagcttc ggtggtggtt tcacctttca gggttttacc gttcaggata
      181 agtttgtact gggtaccatt gaaacaatta gaagctatga agccattttt gagtgcaaaa
      241 ttatggtcgc gctcaactcc aatgtcatag acattttgtt tgcctaaata tttacgtgtg
      301 gctattttga tattcggcaa attatcaacc cgcatcaaat ccaattcacg ttcaaatatt
      361 tcatcaattg gcaacatttg accatcaaca gtcataaact tatggtcttt tgttgcccga
      421 atcaatgaac catcttccaa acaatactca aacacctctt gttctccgcg atcgtgccat
      481 tgtgctacag gttgtgtata aatatttcca ttattatcaa cgctataaac agtacattcg
      541 atgcgctttt ctacaatttt accaatcggt aataatccat attccatatg tatatctcct
      601 tcttaaagtt aaacaaaatt atttctagcc caaaaaaacg ggtatggaga aacagtagag
      661 agttgcgata aaaagcgtca ggtaggatcc gctaatctta tggataaaaa tgctatggca
      721 tagcaaagtg tgacgccgtg caaataatca atgtggactt ttctgccgtg attatagaca
      781 cttttgttac gcgtttttgt catggctttg g
//
