LOCUS       sequence-103830-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TvoVMAdN11 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSABAD14-98
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             64..86
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             805..822
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgacgctttt tatcgcaact ctctactgtt tctccatacc cgtttttttg ggctagaaat
       61 aattttgttt aactttaaga aggagatata catatggatg gcaagacaat aaaaataaag
      121 gatctataca gttctgagag aaaaaaagaa gataacattg ttgaggctgg ttcgggagaa
      181 gagataatac atctaaaaga tcccattcaa atatattctt atgtggacgg gaccatagtc
      241 aggagcagat caagacttct atacaagggc aagagctctt atcttgtgag gatagaaact
      301 attggcggaa gatcggtaag cgttacacca gttcacaaac tctttgtcct tacggaaaag
      361 ggtatcgaag aggttatggc ctccaaccta aaggtaggcg acatgattgc tgctgtagca
      421 gaaagcgaat ctgaagcaag agactgcgga atgagcgagg aatgcgtgat ggaagcagaa
      481 gtttatacgt cacttgaagc gacattcgat agagtaaagt ctatagcgta cgagaagggt
      541 gattttgatg tatacgatct ttccgtacca gaatacggca ggaactttat aggcggagaa
      601 ggacttctcg tacttcacaa cacggttata ggtacccagt acaaacttat cctgaacggt
      661 aaaaccctga aaggtgaaac caccaccgaa gctgttgacg ctgctaccgc ggaaaaagtt
      721 ttcaaacagt acgctaacga caacggtgtt gacggtgaat ggacctacga cgacgctacc
      781 aaaacctaca ccgtaacgga attccatcat catcatcatc attaagcttg gctgttttgg
      841 cggatgagag aagattttca gcctgataca gatt
//
