LOCUS       sequence-103831-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TvoVMAdN11 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSABAD14-98
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..28)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             747..769
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gccaagctta atgatgatga tgatgatgga attccgttac ggtgtaggtt ttggtagcgt
       61 cgtcgtaggt ccattcaccg tcaacaccgt tgtcgttagc gtactgtttg aaaacttttt
      121 ccgcggtagc agcgtcaaca gcttcggtgg tggtttcacc tttcagggtt ttaccgttca
      181 ggataagttt gtactgggta cctataaccg tgttgtgaag tacgagaagt ccttctccgc
      241 ctataaagtt cctgccgtat tctggtacgg aaagatcgta tacatcaaaa tcacccttct
      301 cgtacgctat agactttact ctatcgaatg tcgcttcaag tgacgtataa acttctgctt
      361 ccatcacgca ttcctcgctc attccgcagt ctcttgcttc agattcgctt tctgctacag
      421 cagcaatcat gtcgcctacc tttaggttgg aggccataac ctcttcgata cccttttccg
      481 taaggacaaa gagtttgtga actggtgtaa cgcttaccga tcttccgcca atagtttcta
      541 tcctcacaag ataagagctc ttgcccttgt atagaagtct tgatctgctc ctgactatgg
      601 tcccgtccac ataagaatat atttgaatgg gatcttttag atgtattatc tcttctcccg
      661 aaccagcctc aacaatgtta tcttcttttt ttctctcaga actgtataga tcctttattt
      721 ttattgtctt gccatccata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag
      781 cccaaaaaaa cgggtatgga gaaacagtag agagttgcga
//
