LOCUS       sequence-103834-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TvoVMAdC16 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-143
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..358
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
ORIGIN
        1 attttgttta actttaataa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcactagt gactcagaag tcaatcaaga agctaagcca gaggtcaagc cagaagtcaa
      121 gcctgagact cacatcaatt taaaggtgtc cgatggatct tcagaaatct tctttaagat
      181 caaaaagacc actcctttaa gaaggctgat ggaagcgttc gctaaaagac agggtaagga
      241 aatggactcc ttaagattct tgtacgacgg tattagaatt caagctgatc agacccctga
      301 agatttggac atggaggata acgatattat tgagactcac agagaacaga ttggtggatc
      361 caagtgcgta tcaggtgaaa caccagttta ccttgcggat ggcaagacaa taaaaataaa
      421 ggatctatac agttctgaga gaaaaaaaga agataacatt gttgaggctg gttcgggaga
      481 agagataata catctaaaag atcccattca aatatattct tatgtggacg ggaccatagt
      541 caggagcaga tcaagacttc tatacaaggg caagagctct tatcttgtga ggatagaaac
      601 tattggcgga agatcggtaa gcgttacacc agttcacaaa ctctttgtcc ttacggaaaa
      661 gggtatcgaa gaggttatgg cctccaacct aaaggtaggc gacatgattg ctgctgtagc
      721 agaaagcgaa tctgaagcaa gagactgcgg aatgagcgag gaatgcgtga tggaagcaga
      781 agtttatacg tcacttgaag cgacattcga tagagtaaag tctatagcgt ac
//
