LOCUS       sequence-103837-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TvoVMAdC16 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-143
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..60)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(605..889)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="SEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRL
                     MEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
ORIGIN
        1 agctgcgcta gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt
       61 accagactcg agtgcggccg caagcttatt ctggtacgga aagatcgtat acatcaaaat
      121 cacccttctc gtacgctata gactttactc tatcgaatgt cgcttcaagt gacgtataaa
      181 cttctgcttc catcacgcat tcctcgctca ttccgcagtc tcttgcttca gattcgcttt
      241 ctgctacagc agcaatcatg tcgcctacct ttaggttgga ggccataacc tcttcgatac
      301 ccttttccgt aaggacaaag agtttgtgaa ctggtgtaac gcttaccgat cttccgccaa
      361 tagtttctat cctcacaaga taagagctct tgcccttgta tagaagtctt gatctgctcc
      421 tgactatggt cccgtccaca taagaatata tttgaatggg atcttttaga tgtattatct
      481 cttctcccga accagcctca acaatgttat cttctttttt tctctcagaa ctgtatagat
      541 cctttatttt tattgtcttg ccatccgcaa ggtaaactgg tgtttcacct gatacgcact
      601 tggatccacc aatctgttct ctgtgagtct caataatatc gttatcctcc atgtccaaat
      661 cttcaggggt ctgatcagct tgaattctaa taccgtcgta caagaatctt aaggagtcca
      721 tttccttacc ctgtctttta gcgaacgctt ccatcagcct tcttaaagga gtggtctttt
      781 tgatcttaaa gaagatttct gaagatccat cggacacctt taaattgatg tgagtctcag
      841 gcttgacttc tggcttgacc tctggcttag cttcttgatt gacttctga
//
