LOCUS       sequence-103838-        2376 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Zetsche B, Volz SE, Zhang F
  TITLE     A split-Cas9 architecture for inducible genome editing and 
            transcription modulation.
  JOURNAL   Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
  PUBMED    25643054
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149."
COMMENT     Sequence Label: PX851
FEATURES             Location/Qualifiers
     source          1..2376
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             233..1834
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMR"
     CDS             1880..2149
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     primer_bind     complement(2177..2194)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2183..2294
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg
       61 ggcgggacgg cccttctcct ccgggctgta attagctgag caagaggtaa gggtttaagg
      121 gatggttggt tggtggggta ttaatgttta attacctgga gcacctgcct gaaatcactt
      181 tttttcaggt tggaccggtg ccaccatgct agatttagct agcttaatcc tcgacaagaa
      241 gtacagcatc ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga
      301 gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa
      361 gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct
      421 gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga
      481 gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc
      541 cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga
      601 cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga
      661 cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt
      721 ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct
      781 gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag
      841 cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa
      901 tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc tgattgccct
      961 gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact
     1021 gcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga
     1081 ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga
     1141 catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag
     1201 atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc
     1261 tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga
     1321 cggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga
     1381 cggcaccgag gaactgctcg tgaagctgaa cagagaggac ctgctgcgga agcagcggac
     1441 cttcgacaac ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg
     1501 gcggcaggaa gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct
     1561 gaccttccgc atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg
     1621 gatgaccaga aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa
     1681 gggcgcttcc gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa
     1741 cgagaaggtg ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct
     1801 gaccaaagtg aaatacgtga ccgagggaat gagaggtggc ggtggctcgg gtggcggtgg
     1861 ctcgggtggc ggtggctcgg agatgtggca cgagggcctg gaggaggcca gcagactgta
     1921 cttcggcgag agaaacgtga agggcatgtt cgaggtgctg gagcccctgc acgccatgat
     1981 ggagagaggc ccccagaccc tgaaggagac cagcttcaac caggcctacg gcagagacct
     2041 gatggaggcc caggagtggt gcagaaagta catgaagagc ggcaacgtga aggacctgac
     2101 ccaggcctgg gacctgtact accacgtgtt cagaagaatc agcaagcagt aagaattcct
     2161 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     2221 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     2281 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     2341 caggacagca agggggagga ttgggaagag aatagc
//
