LOCUS       sequence-103842-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PhoRadAdC14 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-166
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..362
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
ORIGIN
        1 aataattttg tttaacttta ataaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcac tagtgactca gaagtcaatc aagaagctaa gccagaggtc aagccagaag
      121 tcaagcctga gactcacatc aatttaaagg tgtccgatgg atcttcagaa atcttcttta
      181 agatcaaaaa gaccactcct ttaagaaggc tgatggaagc gttcgctaaa agacagggta
      241 aggaaatgga ctccttaaga ttcttgtacg acggtattag aattcaagct gatcagaccc
      301 ctgaagattt ggacatggag gataacgata ttattgagac tcacagagaa cagattggtg
      361 gatccgggaa gtgctttgct agggataccg aagtttatta tgaaaacgat actgtaccac
      421 acatggaatc aattgaggag atgtatagta aatacgcctc gatgaatggg gaattaccat
      481 tcgataatgg ttacgcagtt ccattagata atgtctttgt gtacacgttg gacatcgcta
      541 gtggtgaaat taagaaaacg agggcttcat acatctatcg cgagaaggtt gagaagctca
      601 ttgagataaa actatccagt ggatactccc ttaaggttac tccctctcac ccagttctcc
      661 tctttagaga tggcctgcaa tgggtccccg ctgctgaagt taaacctgga gatgttgttg
      721 ttggtgttag ggaagaggta ctgagaagaa gaataatatc caaaggagaa cttgaattcc
      781 atgaggtttc ctcggtaagg ataatagatt acaataactg ggtctacgac cttgtaattc
      841 cggaataagc ttgcggccgc actcgagtct ggtaaagaaa ccgctgctgc gaaa
//
