LOCUS       sequence-103845-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PhoRadAdC14 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-166
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..58)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(570..860)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             complement(867..884)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctgcgctagt agacgagtcc atgtgctggc gttcaaattt cgcagcagcg gtttctttac
       61 cagactcgag tgcggccgca agcttattcc ggaattacaa ggtcgtagac ccagttattg
      121 taatctatta tccttaccga ggaaacctca tggaattcaa gttctccttt ggatattatt
      181 cttcttctca gtacctcttc cctaacacca acaacaacat ctccaggttt aacttcagca
      241 gcggggaccc attgcaggcc atctctaaag aggagaactg ggtgagaggg agtaacctta
      301 agggagtatc cactggatag ttttatctca atgagcttct caaccttctc gcgatagatg
      361 tatgaagccc tcgttttctt aatttcacca ctagcgatgt ccaacgtgta cacaaagaca
      421 ttatctaatg gaactgcgta accattatcg aatggtaatt ccccattcat cgaggcgtat
      481 ttactataca tctcctcaat tgattccatg tgtggtacag tatcgttttc ataataaact
      541 tcggtatccc tagcaaagca cttcccggat ccaccaatct gttctctgtg agtctcaata
      601 atatcgttat cctccatgtc caaatcttca ggggtctgat cagcttgaat tctaataccg
      661 tcgtacaaga atcttaagga gtccatttcc ttaccctgtc ttttagcgaa cgcttccatc
      721 agccttctta aaggagtggt ctttttgatc ttaaagaaga tttctgaaga tccatcggac
      781 acctttaaat tgatgtgagt ctcaggcttg acttctggct tgacctctgg cttagcttct
      841 tgattgactt ctgagtcact agtgctgtga tgatgatgat gatggct
//
