LOCUS       sequence-103848-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NpuDnaEN11 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-756
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..59)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(124..414)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             complement(421..438)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    494..518
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(518..537)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(519..537)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        583..660
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(680..699)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gctgcgctag tagacgagtc catgtgctgg cgttcaaatt tcgcagcagc ggtttcttta
       61 ccagactcga gtgcggccgc aagcttatac tgtcaatatt tccgtttcat agcttaaaca
      121 ggatccacca atctgttctc tgtgagtctc aataatatcg ttatcctcca tgtccaaatc
      181 ttcaggggtc tgatcagctt gaattctaat accgtcgtac aagaatctta aggagtccat
      241 ttccttaccc tgtcttttag cgaacgcttc catcagcctt cttaaaggag tggtcttttt
      301 gatcttaaag aagatttctg aagatccatc ggacaccttt aaattgatgt gagtctcagg
      361 cttgacttct ggcttgacct ctggcttagc ttcttgattg acttctgagt cactagtgct
      421 gtgatgatga tgatgatggc tgctgcccat ggtatatctc cttattaaag ttaaacaaaa
      481 ttatttctac aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt
      541 tcctaatgca ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgaatggcgc
      601 aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat
      661 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
      721 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
      781 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
      841 tcgtt
//
