LOCUS       sequence-103852-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NpuDnaEN35 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-218
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..366
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             503..547
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(585..603)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      599..646
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgtaaataat tttgtttaac tttaataagg agatatacca tgggcagcag ccatcatcat
       61 catcatcaca gcactagtga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca
      121 gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agaaatcttc
      181 tttaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag
      241 ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag
      301 acccctgaag atttggacat ggaggataac gatattattg agactcacag agaacagatt
      361 ggtggatcct gtttaagcta tgaaacggaa atattgacag tagaatatgg attattaccg
      421 attggtaaaa ttgtagaaaa gcgcatcgaa tgtactgttt atagcgttga taattaagct
      481 tgcggccgca ctcgagtctg gtaaagaaac cgctgctgcg aaatttgaac gccagcacat
      541 ggactcgtct actagcgcag cttaattaac ctaggctgct gccaccgctg agcaataact
      601 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aacctcaggc
      661 atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata
      721 gacataagcg gctatttaac gaccctgccc tgaaccgacg acaagctgac gaccgggtct
      781 ccgcaagtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      841 tacattcaaa tatgtatccg ctcatgaatt aattcttag
//
