LOCUS       sequence-103859-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PhoRadAN38 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-459
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..358
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             510..554
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(592..610)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      606..653
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attttgttta actttaataa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcactagt gactcagaag tcaatcaaga agctaagcca gaggtcaagc cagaagtcaa
      121 gcctgagact cacatcaatt taaaggtgtc cgatggatct tcagaaatct tctttaagat
      181 caaaaagacc actcctttaa gaaggctgat ggaagcgttc gctaaaagac agggtaagga
      241 aatggactcc ttaagattct tgtacgacgg tattagaatt caagctgatc agacccctga
      301 agatttggac atggaggata acgatattat tgagactcac agagaacaga ttggtggatc
      361 cgggaagtgc tttgctaggg ataccgaagt ttattatgaa aacgatactg taccacacat
      421 ggaatcaatt gaggagatgt atagtaaata cgcctcgatg aatggggaat taccattcga
      481 ttaagcttgc ggccgcactc gagtctggta aagaaaccgc tgctgcgaaa tttgaacgcc
      541 agcacatgga ctcgtctact agcgcagctt aattaaccta ggctgctgcc accgctgagc
      601 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaac
      661 ctcaggcatt tgagaagcac acggtcacac tgcttccggt agtcaataaa ccggtaaacc
      721 agcaatagac ataagcggct atttaacgac cct
//
