LOCUS       sequence-103869-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NpuDnaB(delta283)dC39 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-259
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..58)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(387..677)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             complement(684..701)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    757..781
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(781..800)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(782..800)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctgcgctagt agacgagtcc atgtgctggc gttcaaattt cgcagcagcg gtttctttac
       61 cagactcgag tgcggccgca agcttaattt cttggtaaac tgagatgttc tttaggagtt
      121 aattcatcaa gtcttttcca gccattaatt gttaaaaact tgtgattacc tgtggcacgg
      181 attttccgcc ccaatcgagt tgtcaaagta aacagaggct ttataccagt cgaaaaagca
      241 ttgctaacaa ttgccttttc tagctgcatt gtagcttcgt tcaatgccca aactgcaaaa
      301 ccagatttac ctactaattc cttaattggc acttgcagtc cgctatctac taatgtcacc
      361 agactatcgc ctgctaaaca accggatcca ccaatctgtt ctctgtgagt ctcaataata
      421 tcgttatcct ccatgtccaa atcttcaggg gtctgatcag cttgaattct aataccgtcg
      481 tacaagaatc ttaaggagtc catttcctta ccctgtcttt tagcgaacgc ttccatcagc
      541 cttcttaaag gagtggtctt tttgatctta aagaagattt ctgaagatcc atcggacacc
      601 tttaaattga tgtgagtctc aggcttgact tctggcttga cctctggctt agcttcttga
      661 ttgacttctg agtcactagt gctgtgatga tgatgatgat ggctgctgcc catggtatat
      721 ctccttatta aagttaaaca aaattatttc tacaggggaa ttgttatccg ctcacaattc
      781 ccctatagtg agtcgtatta atttcctaat gcaggagtcg cat
//
