LOCUS       sequence-103873-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TvoVMAdC7 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-HY176
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..365
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
ORIGIN
        1 gtaaataatt ttgtttaact ttaataagga gatataccat gggcagcagc catcatcatc
       61 atcatcacag cactagtgac tcagaagtca atcaagaagc taagccagag gtcaagccag
      121 aagtcaagcc tgagactcac atcaatttaa aggtgtccga tggatcttca gaaatcttct
      181 ttaagatcaa aaagaccact cctttaagaa ggctgatgga agcgttcgct aaaagacagg
      241 gtaaggaaat ggactcctta agattcttgt acgacggtat tagaattcaa gctgatcaga
      301 cccctgaaga tttggacatg gaggataacg atattattga gactcacaga gaacagattg
      361 gtgggtccaa gtgcgtatca ggtgaaacac cagtttacct tgcggatggc aagacaataa
      421 aaataaagga tctatacagt tctgagagaa aaaaagaaga taacattgtt gaggctggtt
      481 cgggagaaga gataatacat ctaaaagatc ccattcaaat atattcttat gtggacggga
      541 ccatagtcag gagcagatca agacttctat acaagggcaa gagctcttat cttgtgagga
      601 tagaaactat tggcggaaga tcggtaagcg ttacaccagt tcacaaactc tttgtcctta
      661 cggaaaaggg tatcgaagag gttatggcct ccaacctaaa ggtaggcgac atgattgctg
      721 ctgtagcaga aagcgaatct gaagcaagag actgcggaat gagcgaggaa tgcgtgatgg
      781 aagcagaagt ttatacgtca cttgaagcga cattcgatag agtaaagtct atagcgtacg
      841 agaagggtga ttttgatgta tacgatcttt ccgtaccaga atacggcagg aactttatag
      901 gcggagaata agcttgcggc cgcactcgag
//
