LOCUS       sequence-103876-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express C-terminal Ubl4A K48R, for making the stable cell line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Liu Y, Soetandyo N, Lee JG, Liu L, Xu Y, Clemons WM Jr, Ye Y
  TITLE     USP13 antagonizes gp78 to maintain functionality of a chaperone in 
            ER-associated degradation.
  JOURNAL   Elife. 2014;3:e01369. doi: 10.7554/eLife.01369. Epub 2014 Jan 14.
  PUBMED    24424410
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014"; volume: "3:e01369. doi"; pages: " 10.7554/eLife.01369.
            Epub 2014 Jan 14"
COMMENT     Sequence Label: pcDNA 5/FR/TO-Ubl4A K48R
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(92..139)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     271..290
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    423..647
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     636..653
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(750..773)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(792..821)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga
       61 cgtcgcggtg agttcaggct ttttggccaa ggaagttcct atactttcta gagaatagga
      121 acttcggaat aggaacttct aggtacgtga accatcaccc taatcaagtt ttttggggtc
      181 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
      241 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
      301 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
      361 gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt ccgcctcaga
      421 agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt
      481 gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc
      541 aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg
      601 cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca
      661 gcgggtttaa acgggccctc tagactcgag cggccgccac tgtgctggat atctgcagaa
      721 ttccaccaca ctggactagt ggatccctac ttatcgtcgt catccttgta atccaggata
      781 tcatttgctg ccagatcctc ttctgagatg agtttctgct cgagcggccg cgtacgcgtt
      841 ttggag
//
