LOCUS       sequence-103879-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NpuDnaB(delta283)N11 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-HY100
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..362
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             436..480
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(518..536)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      532..579
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aataattttg tttaacttta ataaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcac tagtgactca gaagtcaatc aagaagctaa gccagaggtc aagccagaag
      121 tcaagcctga gactcacatc aatttaaagg tgtccgatgg atcttcagaa atcttcttta
      181 agatcaaaaa gaccactcct ttaagaaggc tgatggaagc gttcgctaaa agacagggta
      241 aggaaatgga ctccttaaga ttcttgtacg acggtattag aattcaagct gatcagaccc
      301 ctgaagattt ggacatggag gataacgata ttattgagac tcacagagaa cagattggtg
      361 gatccggttg tttagcaggc gatagtctgg tgacattagt agatagctaa gcttgcggcc
      421 gcactcgagt ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg
      481 tctactagcg cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa
      541 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag
      601 aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa
      661 gcggctattt aacgaccctg ccctgaaccg acgacaagct gacgaccggg tctccgcaag
      721 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
      781 aaatatgtat ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact
      841 gcaatttatt catatca
//
