LOCUS       sequence-103880-        1143 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CrBlue is a synthetic chimera comprising the blue opsin backbone 
            with the intracellular loops and the C terminus of the jellyfish 
            opsin, created to introduce spectral selectivity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Karunarathne WK, Giri L, Kalyanaraman V, Gautam N
  TITLE     Optically triggering spatiotemporally confined GPCR activity in a 
            cell and programming neurite initiation and extension.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1565-74. doi: 
            10.1073/pnas.1220697110. Epub 2013 Mar 11.
  PUBMED    23479634
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220697110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-04-23- 23"; volume: "110"; issue: "17"; pages: 
            "E1565-74"
COMMENT     Sequence Label: CrBlue ---- pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..1143
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(796..813)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtggaattca attccaccat gcgcaagatg tccgaggagg agttctacct gttcaagaac
       61 atctcgagcg tcggcccttg ggacgggccc cagtaccaca tcgctcctgt ctgggctttc
      121 tacctccagg ctgctttcat ggggaccgtg ttcctcatcg gtttccctct gaatgccatg
      181 gtgctcgtgg ccttttaccg cttacgacat aaattggctt ttttaaacta catcctggtg
      241 aacgtgtcct tcggtggatt cctgctgtgt atcttctccg tgttccctgt gttcgtggct
      301 agctgcaacg gttacttcgt gttcggacgt cacgtgtgtg ctctggaggg tttcctaggt
      361 accgtggctg gtctggtcac cgggtggtct ctggccttcc tggccttcga acgctacatc
      421 gtcatatgca gacctttcgt ggcaactgcg attcacggat caatgagaaa tgcgttaacc
      481 gtagtactgg ctacctggac aatcggtatc ggtgtatcga tccctccctt cttcggttgg
      541 tctcgcttca ttccggaagg ccttcagtgc agctgtggcc ctgactggta caccgtgggt
      601 acaaagtacc gatcggagag ctacacctgg ttcctgttca tcttctgctt catcgtgcct
      661 ctgagcctga tctgcttctc ctacaccttg gttcaaggag agatgaagaa tatgcgaggt
      721 cgtgcagcac agttgtttgg ttcagaatcc gaggctgcac taaagaatat caaggccgaa
      781 aagcgacaca caagaatggt ggtggtgatg gtgggatcct tctgcgtgtg ctacgtaccc
      841 tacgctgctt tcgccatgta catggtgaac aacaggaacc acggtttaga tctgcgactg
      901 gtgacaatcc cttcgttctt ctccaagtcc gcatcgattt acaaccctat catctactgc
      961 ttcatgaaca agcagttcca aagagctgta ttacgtggtg tttgtggaag aattgtcggt
     1021 ggtaatgcca ttgctccttc cagtacggct gttgaaccta acgagacctc ccaggtggct
     1081 cctgcttgaa agcttgcggc cgctcgagtc tagagggccc gttaaacccg ctgatcagcc
     1141 tcg
//
