LOCUS       sequence-103882-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NpuDnaB(delta283)N11 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-HY100
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..58)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(132..422)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             complement(429..446)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    502..526
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(526..545)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(527..545)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        591..668
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(688..707)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ctgcgctagt agacgagtcc atgtgctggc gttcaaattt cgcagcagcg gtttctttac
       61 cagactcgag tgcggccgca agcttagcta tctactaatg tcaccagact atcgcctgct
      121 aaacaaccgg atccaccaat ctgttctctg tgagtctcaa taatatcgtt atcctccatg
      181 tccaaatctt caggggtctg atcagcttga attctaatac cgtcgtacaa gaatcttaag
      241 gagtccattt ccttaccctg tcttttagcg aacgcttcca tcagccttct taaaggagtg
      301 gtctttttga tcttaaagaa gatttctgaa gatccatcgg acacctttaa attgatgtga
      361 gtctcaggct tgacttctgg cttgacctct ggcttagctt cttgattgac ttctgagtca
      421 ctagtgctgt gatgatgatg atgatggctg ctgcccatgg tatatctcct tattaaagtt
      481 aaacaaaatt atttctacag gggaattgtt atccgctcac aattccccta tagtgagtcg
      541 tattaatttc ctaatgcagg agtcgcataa gggagagcgt cgagatcccg gacaccatcg
      601 aatggcgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg
      661 tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc
      721 agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag
      781 tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg
      841 gcaaacagtc gttgctgatt ggcgttgcca cctccagtct gg
//
