LOCUS       sequence-103884-         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus expression vector for secretion with N-terminal Honeybee
            melittin signal and C-terminal 8xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  TITLE     Arrowsmith Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAB-LIC C
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     60..122
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
                     /translation="MKFLVNVALVFMVVYISYIYA"
     protein_bind    complement(143..176)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        191..636
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 cctataaata ttccggatta ttcataccgt cccaccatcg ggcgcggatc agatctaaaa
       61 tgaaattctt agtcaacgtt gcccttgttt ttatggtcgt atacatttct tacatctatg
      121 cggccgctct cctgaaagat ccataacttc gtatagcata cattatacga agttatgcgg
      181 ccgcgacgtc cacatatacc tgccgttcac tattatttag tgaaatgaga tattatgata
      241 ttttctgaat tgtgattaaa aaggcaactt tatgcccatg caacagaaac tataaaaaat
      301 acagagaatg aaaagaaaca gatagatttt ttagttcttt aggcccgtag tctgcaaatc
      361 cttttatgat tttctatcaa acaaaagagg aaaatagacc agttgcaatc caaacgagag
      421 tctaatagaa tgaggtcgaa aagtaaatcg cgcgggtttg ttactgataa agcaggcaag
      481 acctaaaatg tgtaaagggc aaagtgtata ctttggcgtc accccttaca tattttaggt
      541 ctttttttat tgtgcgtaac taacttgcca tcttcaaaca ggagggctgg aagaagcaga
      601 ccgctaacac agtacataaa aaaggagaca tgaacgatga acatcaaaaa gtttgcaaaa
      661 caagcaacag tattaacctt tactaccgca ctgctggcag gaggcgcaac tcaagcgttt
      721 gcg
//
