LOCUS       sequence-103887-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  TITLE     Arrowsmith Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET21a-LIC
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..31
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             56..76
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(106..139)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        154..599
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 agatatacat atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa
       61 cctgtacttc caatccataa gctagcttct cctcctgaaa gatccataac ttcgtatagc
      121 atacattata cgaagttatg cggccgcgac gtccacatat acctgccgtt cactattatt
      181 tagtgaaatg agatattatg atattttctg aattgtgatt aaaaaggcaa ctttatgccc
      241 atgcaacaga aactataaaa aatacagaga atgaaaagaa acagatagat tttttagttc
      301 tttaggcccg tagtctgcaa atccttttat gattttctat caaacaaaag aggaaaatag
      361 accagttgca atccaaacga gagtctaata gaatgaggtc gaaaagtaaa tcgcgcgggt
      421 ttgttactga taaagcaggc aagacctaaa atgtgtaaag ggcaaagtgt atactttggc
      481 gtcacccctt acatatttta ggtctttttt tattgtgcgt aactaacttg ccatcttcaa
      541 acaggagggc tggaagaagc agaccgctaa cacagtacat aaaaaaggag acatgaacga
      601 tgaacatcaa aaagtttgca aaacaagcaa cagtattaac ctttactacc gcactgctgg
      661 caggaggcgc aactcaagcg
//
