LOCUS       sequence-103888-         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  TITLE     Arrowsmith Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET21a-LIC
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa gcttgtcgac
       61 ggagctcgaa ttcggatccg tatccacctt tacttactag taaactcctc gaaaagtgcc
      121 acctgacgtc aatgccaata ggatatcggc attttctttt gcgtttttat ttgttaactg
      181 ttaattgtcc ttgttcaagg atgctgtctt tgacaacaga tgttttcttg cctttgatgt
      241 tcagcaggaa gctaggcgca aacgttgatt gtttgtctgc gtagaatcct ctgtttgtca
      301 tatagcttgt aatcacgaca ttgtttcctt tcgcttgagg tacagcgaag tgtgagtaag
      361 taaaggttac atcgttagga tcaagatcca tttttaacac aaggccagtt ttgttcagcg
      421 gcttgtatgg gccagttaaa gaattagaaa cataaccaag catgtaaata tcgttagacg
      481 taatgccgtc aatcgtcatt tttgatccgc gggagtcagt gaacaggtac catttgccgt
      541 tcattttaaa gacgttcgcg cgttcaattt catctgttac tgtgttagat gcaatcagcg
      601 gtttcatcac ttttttcagt gtgtaatcat cgtttagctc aatcataccg agagcgccgt
      661 ttgctaactc agccgtgcgt tttttatcgc tttgcag
//
