LOCUS       sequence-10389-e        9399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Kim JG, Armstrong RC, v Agoston D, Robinsky A, Wiese C, Nagle J, 
            Hudson LD
  TITLE     Myelin transcription factor 1 (Myt1) of the oligodendrocyte lineage,
            along with a closely related CCHC zinc finger, is expressed in 
            developing neurons in the mammalian central nervous system.
  JOURNAL   J Neurosci Res. 1997 Oct 15. 50(2):272-90.
  PUBMED    9373037
REFERENCE   2  (bases 1 to 9399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Res. 1997 Oct 15. 50(2):272-90."
COMMENT     Sequence Label: pmMyt1
FEATURES             Location/Qualifiers
     source          1..9399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     239..256
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(3721..3732)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(5855..5874)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5856..5874)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5881..5898)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5881..5897)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5890..5912)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6039..6419
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6126..6145)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6336..6357
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             6787..7581
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SRLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVIHGDACLPNIMVENGRFSGFNDCGRLGVADRYQDIA
                     LDTRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6841..6860)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             join(7599..7667,7668..8459)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCDTLLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLID*MEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8630..9218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9119..9138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9372..9389
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
      301 ctagtaacgg ccgccagtgt gctggaattc ggcttaacac accacgaggt actgtgagtc
      361 caacgtgttt cctgaggaag gagggtcaca gtattggggt atcaagatcc tgggcagctt
      421 ggccagcgag tccattgccc agaactgacc atggccagca ctccctcaca gcaacatgaa
      481 tcatccatga gaacctccac aaatgcaaca caagaaacac cagagcacac acatctgcac
      541 tacacttaaa taataataaa tacagaattc catttgctgt tattggcctg acaaaaggaa
      601 cccaaaggga aaaagaaaac caaacacctt tctctctggt ggaccaatgg ggagaaaaac
      661 ccaaattatc aaaattcttc agttacaaaa tactttttta aaaaattcca ttattaaaag
      721 aataaataac aaaatatata aaaaataact cggttgcatc aaaattataa ctagaagtta
      781 tatcaagatt ctttgtggcc tatcttatag tcaaataaat gtttttgttt caatttaact
      841 tatttaactc ttatggtgcc ctagtgtgtc atctagttga tatagtataa aataaataaa
      901 taaaaataat aaatagcaaa cattaacata agagggtaag aatataaata cttatgacaa
      961 aattaaataa tttacagaat aaactaacta ctttcagtgt cttccaggaa gtgcaatttc
     1021 tctactttta aatactaatt tggaattgca gtcacaattg acttggactc tatttacaca
     1081 gtaagaacat tgtgttttta acacattaaa ttaaaattta tccttttcac atcttaaatg
     1141 aacctataaa gaagtgaaat cttggtggtc tccctgtaac aatttaccac tggtctcctg
     1201 ccccactgca gtcaccttgg atacaaggag caaacctatg caggaaaaga tacaatgatg
     1261 ggtccagcac ctggatggag gtacggaaac cagaggtttg gcccagtggt cttggactta
     1321 gagcccaagc caggggttaa aaactggagc caggagaaag cagatctgca cagcttagag
     1381 gcatctgctg cccaagtaag tggccgattt ttcagggcct ccaactgaaa acaaaaaaaa
     1441 acctccagct gactttctat gatgctcatc aactgtgtag actgccatct gggaagcctg
     1501 ttcctagatt cacctctgaa gcagagtatt gtgcaccttt aacacaaagt tgaattaaat
     1561 gaaaacacaa aacatacttg tcatgtgggc ttcagtgagc atagtcagaa acctaggcat
     1621 gccctcctgc tgagactaac acccagcagg gccatggctg ccctcccaac cttgacaaac
     1681 aatggaatac tgtggctgca aactccaaaa cttccctgag tgagaacctt gtccatgagc
     1741 ttccaccgga gactttgagc tgctcttcag acttctttta aaaaaaacaa aaaacaaaga
     1801 acaaaaacaa aaataagtca ctttgcaata cttcagtgtc cccaggcact cacacagaga
     1861 ggtcttcatg cctgggccta atgaggacat cataggagac actgattccc aggctcccct
     1921 ttgggtaagc tgcacaaggc tgcacccagg cttccaatta gaagtagcta gaagtcaggg
     1981 tccttgctgt gaggagcaga gcagactcct gtgtgcagtg cagcctagac ctgaatgccc
     2041 ctcacagctt gcttgatcgt ttccagaagg cctttgttct ccggattctg gtagcagtcc
     2101 tgattggagt acatgtcagt gagggtgttc acataggcgt cgaaattctg ttcacatatt
     2161 ggttccatgt gtggaaggcg aatattggca agactttgga tgagggcttg gctaagcccg
     2221 gacaattcca gaaacagggc ctcattctgc tcctcaatga gcttgttctc ctcctcgatg
     2281 ttcttcaggt tcctctccat ggacgagatc tgagactgca gctgcaccat ggcagcctcc
     2341 attccggaat tggactcatt taagtctcgt atctcctgat ttagctgctt gatctcctca
     2401 tcattctcca gcacatcact tgtcttgaac tttgggctga gaatctcatc ccctgagagt
     2461 tttcctttct ttccagcaaa ggttgctctg ggacagccag acaaacttcg gtgggtgagg
     2521 aaactgccgt tggcatggcc agagccgtcg caacctgggg tggggcaagt agggccctca
     2581 ttcttcagcg acttccacga gaaagatgaa ccattgagag ctccctcttt ctgcctccga
     2641 gcagccagtg ggcagccaga agcactcctg tgagaggcat atttgccgct gatgtggccg
     2701 agccccacac agcctggaac tgggcacttc atcaactcgg ggtcctcctt gtcatccttg
     2761 gtaggcgcca ccctgagtcc actcttcttg gcacgtgggc agcctgacaa actccggtgt
     2821 gaagcatagt tccctgttat gtggccagag ccatcacagc caggtgtggg gcacttgagg
     2881 tcagcagagt gggcagccat gaggtttctg aggctcttgt cagcaagagg gcaaccagag
     2941 aggctgcgat gggaggcata gttcccggtg atatggccac tgccgtcaca gccgggggtg
     3001 ggacaggtga gaagctcctt ctttatgtcc ttggaaagaa acttcagctt aaagttggtt
     3061 agggtgactt cccctggata cttccgctcc tcaaagttct cttctgacac ctcctgatca
     3121 tctgcttcag aagaagcaaa agagtgtgct gctggctctg actcctcagg ctcttcctct
     3181 cgaagccgac taggtttggt gtagtctaga ggacggtccc actcatcctg gcgagaggcc
     3241 tggctggact ggggtgaggt catagagctg ctgggggcac tggtactgct ctgacgctga
     3301 gagacaccag gagacttgac accagggctg ctactgcagc tgctactgct ggggaaagtg
     3361 ttttcccgct tgcgatgttt atgcatgctc aagtccagag ttccattctc atctacctca
     3421 atgtccacag ccttgctggg gaggtcctgt ggctttgtgc tgaggttctc tggcatctcc
     3481 caacatcgag tggagaggtt caggatggct gtggcagcca tgtgtgcagc ctcagcatca
     3541 tgagagtaat cgaagcattg gaaggctttg ggtgaggttt cgctggtctg aatctttggg
     3601 gcaagcatac gtttgccaaa aacctgagca tcaaaacttg cgtagtcaaa ggtgactttg
     3661 gagaacttct ccagctcctt ggccaagttg gccctgggcg tggcgggagc cacattgggt
     3721 ctgtagctcc cataaggtgg aacctcaagc tgcttcacaa aacacatggg cctgaggatc
     3781 cgatctgaat tggagttatt tttgggagga tctcctgtct gcaattgctg cttctcatga
     3841 gatttggcta atttttcagc agcagcaatg ggacatccag ataaacttct gtgagtattg
     3901 cggtagctgt tcacatggcc ttggcccgtg cagccaggag tagggcactt cagtacattt
     3961 tcatgcatgg ccaagatctc tggagggatt ctatccttgt ggggacagcc agacaggctg
     4021 cggtgatgag ggtacaatcc agtaacatgg ccagtgccat cacagccagg tgccggacac
     4081 ttgatttcac gcttctccac cctggaagga tctttgctgt agcagctctt cctcaccacg
     4141 tccaggggct ttgccatttt ccctggctct cctgggccca gatggccctg ctcagcaggt
     4201 ggacagccag actcacagac cgctcgcacc tgctcagcct tcagggcaat ggcctgttcc
     4261 agaaggccta aattcccacg ggtcatcatg tcatacattt ctgattcatc tgtgactgct
     4321 gacttctggg agcgtgaatc ctcatccttg tcatcatcag agcgaacctc cacgatgact
     4381 gagtactcag gtttaggact agatagctct gggcctcgga actcaggaga cgccttctgg
     4441 acagaggtat gggaggtgtc ttccccaaag ataacatttg gagctgcttc ctcctcctcc
     4501 tcctcttcct cttcatcttc ctcctcctct tcttcctcct cctcctcctc ttcttcttct
     4561 ccttcctctc cctcctcctc ctcctcctca tcctcttctt cttcatcttc ttcctcctcc
     4621 tctccctcct cctcatgacc taggactcct ttctgcttac ttgactcttc actaaccata
     4681 tccttcagag actggcggca tggctcctgg gaacgttcac ttgtgacttc aatgacctcc
     4741 tccacatctt ctggctgtat aaagaggtcc ttttcaccct cgtcactgtt gacccccatt
     4801 gcagcctcat cctgaagctg atgaacaaca gtggggctca acttagctac tgagacggaa
     4861 tcctccttca caagggcctc ttcagcaatt tggcccagat ttaggaggga agttgcaatg
     4921 atcccctggt agctactgta gctgctcttg gaaagccaga agggctgctt gtggggttgg
     4981 agtaaaaatg gggcttgatg aggctttttc ctgcataaaa caagaaacaa cggggccttg
     5041 aagagagcat gggggaatgt ttccaggtct ctgctcccaa cagtagccaa ctctccacct
     5101 atccccacct gcccactctc agcactgtag cttagaaaat atggaaatga actgtaagcc
     5161 atcctgtcag ccttctgcct ggattcaaaa accaagaagg aatcaatttt agctagatta
     5221 ctttgatatt tttcagttct tattcctatt ccatccatca cttattttct caaagcagat
     5281 agatctgcat tttatacagt ggaacgtaaa gaaaaaaatc aaatgaaaag tcattttata
     5341 attgaaaatc acttatattt tgacagaaca cgaatttaaa acttctaaac atagtaacag
     5401 atcttactac atgctcatcc tgtagaactc atgaatcagt gttcttagca acagtaggag
     5461 ctagccctac gatataatat cccaagaggc aggtaagtca tagatttttt tctacatgga
     5521 acacaaactt gctttattcc atggaccatg cattcccttt agtcccaaga aggaaatcag
     5581 tgagggctca acatagattg cttcaaaaat atggtaaaac tctacctcaa ccatttaaag
     5641 tcagctgatc ccagaaagtt gggaagtcag cagactctca gatgccttct ttcctctctt
     5701 ggcttctatg agcagcccac cttcagagtc ctgattcacc ctgaagtgag gtatttcatc
     5761 agtacctgct cttggtgtct gagaacaagc cgaattctgc agatatccat cacactggcg
     5821 gccgctcgag catgcatcta gagggcccaa ttcgccctat agtgagtcgt attacaattc
     5881 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     5941 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     6001 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     6061 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6121 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6181 agctctaaat cgggggctcc ctttagggtt ccgatttaga gctttacggc acctcgaccg
     6241 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     6301 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     6361 aacactcaac cctatcgcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     6421 ctattggtta aaaaatgagc tgatttaaca aattcagggc gcaagggctg ctaaaggaac
     6481 cggaacacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac
     6541 tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg
     6601 cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca
     6661 gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg
     6721 ccgccaagga tctgatggcg caggggatca agatctgatc aagagacagg atgaggatcg
     6781 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     6841 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     6901 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     6961 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     7021 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     7081 gggcaggatc tcctgtcatc tcgccttgct cctgccgaga aagtatccat catggctgat
     7141 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     7201 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     7261 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     7321 cccgacggcg aggatctcgt cgtgatccat ggcgatgcct gcttgccgaa tatcatggtg
     7381 gaaaatggcc gcttttctgg attcaacgac tgtggccggc tgggtgtggc ggaccgctat
     7441 caggacatag cgttggatac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     7501 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     7561 cttcttgacg agttcttctg aattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7621 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7681 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7741 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7801 gcacttttaa agttctgcta tgtgatacac tattatcccg tattgacgcc gggcaagagc
     7861 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7921 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7981 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8041 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8101 atgaagccat accaaacgac gagagtgaca ccacgatgcc tgtagcaatg ccaacaacgt
     8161 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8221 gaatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8281 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8341 ggccagatgg taagcgctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8401 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8461 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8521 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8581 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8641 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8701 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8761 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8821 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8881 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8941 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9001 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9061 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9121 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9181 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9241 tacggttcct gggcttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9301 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9361 cgaccgagcg cagcgagtca gtgagcgagg aagcggaag
//
