LOCUS       sequence-103890-         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used for tac promoter regulated high-level expression of cloned, 
            untagged genes in E. Coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  TITLE     Arrowsmith Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTRC-LIC
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(31..36,37..53,54..60)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    68..84
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     73..95
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    complement(135..168)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        183..628
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 cggttctggc aaatattctg aaatgagctg ttgacaatta atcatccggc tcgtataatg
       61 tgtggaattg tgagcggata acaatttcac acaggaaaca gaccatgatt atgagttctc
      121 ctcctgaaag atccataact tcgtatagca tacattatac gaagttatgc ggccgcgacg
      181 tccacatata cctgccgttc actattattt agtgaaatga gatattatga tattttctga
      241 attgtgatta aaaaggcaac tttatgccca tgcaacagaa actataaaaa atacagagaa
      301 tgaaaagaaa cagatagatt ttttagttct ttaggcccgt agtctgcaaa tccttttatg
      361 attttctatc aaacaaaaga ggaaaataga ccagttgcaa tccaaacgag agtctaatag
      421 aatgaggtcg aaaagtaaat cgcgcgggtt tgttactgat aaagcaggca agacctaaaa
      481 tgtgtaaagg gcaaagtgta tactttggcg tcacccctta catattttag gtcttttttt
      541 attgtgcgta actaacttgc catcttcaaa caggagggct ggaagaagca gaccgctaac
      601 acagtacata aaaaaggaga catgaacgat gaacatcaaa aagtttgcaa aacaagcaac
      661 agtattaacc tttactaccg cactgctggc aggaggcgca actcaagcgt ttgcg
//
