LOCUS       sequence-103892-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PhoRadAdC6 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-165
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..366
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
ORIGIN
        1 tgtaaataat tttgtttaac tttaataagg agatatacca tgggcagcag ccatcatcat
       61 catcatcaca gcactagtga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca
      121 gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agaaatcttc
      181 tttaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag
      241 ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag
      301 acccctgaag atttggacat ggaggataac gatattattg agactcacag agaacagatt
      361 ggtggatccg ggaagtgctt tgctagggat accgaagttt attatgaaaa cgatactgta
      421 ccacacatgg aatcaattga ggagatgtat agtaaatacg cctcgatgaa tggggaatta
      481 ccattcgata atggttacgc agttccatta gataatgtct ttgtgtacac gttggacatc
      541 gctagtggtg aaattaagaa aacgagggct tcatacatct atcgcgagaa ggttgagaag
      601 ctcattgaga taaaactatc cagtggatac tcccttaagg ttactccctc tcacccagtt
      661 ctcctcttta gagatggcct gcaatgggtc cccgctgctg aagttaaacc tggagatgtt
      721 gttgttggtg ttagggaaga ggtactgaga agaagaataa tatccaaagg agaacttgaa
      781 ttccatgagg tttcctcggt aaggataata gattacaata actgggtcta cgaccttgta
      841 attccggaaa cccacaactt catagctccc aattaagctt gcggccgcac tcgagtctgg
      901 taaa
//
