LOCUS       sequence-103894-         613 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli pACYC based expression vector with N-terminal Hexa-HIS and a
            thrombin protease cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 613)
  TITLE     Arrowsmith Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pACYC-LIC+
FEATURES             Location/Qualifiers
     source          1..613
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             48..65
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     protein_bind    complement(84..117)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        132..577
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 atatacatat gggcagcagc catcatcatc atcatcacag cagcggcctg gttccgcgtg
       61 gtagtagaga ccctgaaaga tccataactt cgtatagcat acattatacg aagttatgcg
      121 gccgcgacgt ccacatatac ctgccgttca ctattattta gtgaaatgag atattatgat
      181 attttctgaa ttgtgattaa aaaggcaact ttatgcccat gcaacagaaa ctataaaaaa
      241 tacagagaat gaaaagaaac agatagattt tttagttctt taggcccgta gtctgcaaat
      301 ccttttatga ttttctatca aacaaaagag gaaaatagac cagttgcaat ccaaacgaga
      361 gtctaataga atgaggtcga aaagtaaatc gcgcgggttt gttactgata aagcaggcaa
      421 gacctaaaat gtgtaaaggg caaagtgtat actttggcgt caccccttac atattttagg
      481 tcttttttta ttgtgcgtaa ctaacttgcc atcttcaaac aggagggctg gaagaagcag
      541 accgctaaca cagtacataa aaaaggagac atgaacgatg aacatcaaaa agtttgcaaa
      601 acaagcaaca gta
//
