LOCUS       sequence-103895-         597 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli pACYC based expression vector with N-terminal Hexa-HIS and a
            thrombin protease cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 597)
  TITLE     Arrowsmith Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pACYC-LIC+
FEATURES             Location/Qualifiers
     source          1..597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..31)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cagccggatc tcagtggtgg tggtggtggt gctcgagtgc ggccgcaagc ttcgtcatca
       61 cgagaccgaa aagtgccacc tgacgtcaat gccaatagga tatcggcatt ttcttttgcg
      121 tttttatttg ttaactgtta attgtccttg ttcaaggatg ctgtctttga caacagatgt
      181 tttcttgcct ttgatgttca gcaggaagct aggcgcaaac gttgattgtt tgtctgcgta
      241 gaatcctctg tttgtcatat agcttgtaat cacgacattg tttcctttcg cttgaggtac
      301 agcgaagtgt gagtaagtaa aggttacatc gttaggatca agatccattt ttaacacaag
      361 gccagttttg ttcagcggct tgtatgggcc agttaaagaa ttagaaacat aaccaagcat
      421 gtaaatatcg ttagacgtaa tgccgtcaat cgtcattttt gatccgcggg agtcagtgaa
      481 caggtaccat ttgccgttca ttttaaagac gttcgcgcgt tcaatttcat ctgttactgt
      541 gttagatgca atcagcggtt tcatcacttt tttcagtgtg taatcatcgt ttagctc
//
