LOCUS       sequence-103902-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Cyclin B1 with R42A mutation in destruction 
            box to make cyclin B1 non-destructable.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Clute P, Pines J
  TITLE     Temporal and spatial control of cyclin B1 destruction in metaphase.
  JOURNAL   Nat Cell Biol. 1999 Jun;1(2):82-7.
  PUBMED    10559878
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol."; date: "1999-06"; volume: "1(2)"; pages: "82-7"
COMMENT     Sequence Label: (R42A) Cyclin B1-GFP (62)
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             98..127
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctatataagc agagctctct ggctaactga gaacccactg cttactggct tatcgaaatt
       61 aatacgactc actataggga gacccaagct tatcatggag caaaaactta tatctgagga
      121 ggacctttcc atggcgctcc gagccaccag gaactcgaaa attaatgctg aaaataaggc
      181 gaagatcaac atggcaggcg caaagcgcgt tcctacggcc cctgctgcaa cctccaagcc
      241 cggactgagg ccagcaacag ctcttgggga cattggtaac aaagtcagtg aacaactgca
      301 ggccaaaatg cctatgaaga aggaagcaaa accttcagct actggaaaag tcattgataa
      361 aaaactacca aaacctcttg aaaaggtacc tatgctggtg ccagtgccag tgtctgagcc
      421 agtgccagag ccagaacctg agccagaacc tgagcctgtt aaagaagaaa aactttcgcc
      481 tgagcctatt ttggttgata ctgcctctcc aagcccaatg gaaacatctg gatgtgcccc
      541 tgcagaagaa gacctgtgtc aggctttctc tgatgtaatt cttgcagtaa atgatgtgga
      601 tgcagaagat ggagctgatc caaacctttg tagtgaatat gtgaaagata tttatgctta
      661 tctgagacaa cttgaggaag agcaagcagt cagaccaaaa tacctactgg gtcgggaagt
      721 cactggaaac atgagagcca tcctaattga ctggctagta caggttcaaa tgaaattcag
      781 gttgttgcag gagaccatgt acatgactgt ctccattatt gatcggttca tgcagaataa
      841 ttgtgtgccc aagaagatgc tgcagctggt tggtgtcact gccatgttta ttgcaagcaa
      901 atatgaagaa atgtaccctc cagaaattgg tgactttgct tttgtgactg acaacactta
      961 tactaagcac caaatcagac agatggaaat gaagattcta agagctttaa actttggtct
     1021 gggtcggcct ctacctttgc acttcctt
//
