LOCUS       sequence-103903-        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Cyclin B1 with R42A mutation in destruction 
            box to make cyclin B1 non-destructable.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Clute P, Pines J
  TITLE     Temporal and spatial control of cyclin B1 destruction in metaphase.
  JOURNAL   Nat Cell Biol. 1999 Jun;1(2):82-7.
  PUBMED    10559878
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol."; date: "1999-06"; volume: "1(2)"; pages: "82-7"
COMMENT     Sequence Label: (R42A) Cyclin B1-GFP (62)
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(295..315)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          445..510
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(660..680)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatct ctagtcaagg cactatacat caaatatttt tccataattt tcttgtatag
      181 cagtgcagct ttttcctttg tggtgtaaat agcaaagcaa gcaagagttc tattactaaa
      241 cacagcatga ctcaaaaaac ttagcaattc tgaaggaaag tccttggggt cttctacctt
      301 tctcttcttt tttggaggag tagaatgttg agagtcagca gtagcctcat catcactaga
      361 tggcatttct tctgagcaaa acaggttttc ctcattaaag gcattccacc actgctccca
      421 ttcatcagtt ccataggttg gaatctaaaa tacacaaaca attagaatca gtagtttaac
      481 acattataca cttaaaaatt ttatatttac cttagagctt taaatctctg taggtagttt
      541 gtccaattat gtcacaccac agaagtaagg ttccttcaca aagatcctct agctacctag
      601 ctagctggcc aggatcctat ttgtatagtt catccatgcc atgtgtaatc ccagcagccg
      661 ttacaaactc aagaaggacc atgtggtctc tcttttcgtt gggatctttc gaaagggcag
      721 attgtgtgga caggtaatgg ttgtctggta aaaggacagg gtcatcgcca attggagtat
      781 tttgttgata atgatcagcg agttgcacgc cgccgtcttc gatgttgtgg cgggtcttga
      841 agttggcttt gatgccgttc ttttgcttgt cggccatgat gtatacgttg tgggagttgt
      901 agttgtattc caacttgtgg ccgaggatgt ttccgtcctc cttgaaatcg attcccttaa
      961 gctcgatcct gttgacgagg gtgtctccct caaacttgac ttcagcacgt gtcttgtagt
     1021 tcccgtcgtc cttgaagaag atggtcctct cctgcacgta tccctcaggc atggcgctct
     1081 tgaagaagtc gtgccgcttc
//
