LOCUS       sequence-103904-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of CDK1 WT, used for Ips.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  TITLE     Pines Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA5/FRT/TO SBBP-CDK1 WT (2007)
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    39..57
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     67..91
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             182..295
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg
       61 tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      121 cctccataga agacaccggg accgatccag cctccggact ctagcgttta aacttaagct
      181 tatggacgaa aaaaccaccg gttggcgtgg tggtcacgtt gttgaaggtc tggctggtga
      241 actggaacag ctgcgtgctc gtctggaaca ccacccgcag ggtcagcgtg aacccggggg
      301 taccgagctc ggatccatgg aagattatac caaaatagag aaaattggag aaggtaccta
      361 tggagttgtg tataagggta gacacaaaac tacaggtcaa gtggtagcca tgaaaaaaat
      421 cagactagaa agtgaagagg aaggggttcc tagtactgca attcgggaaa tttctctatt
      481 aaaggaactt cgtcatccaa atatagtcag tcttcaggat gtgcttatgc aggattccag
      541 gttatatctc atctttgagt ttctttccat ggatctgaag aaatacttgg attctatccc
      601 tcctggtcag tacatggatt cttcacttgt taagagttat ttataccaaa tcctacaggg
      661 gattgtgttt tgtcactcta gaagagttct tcacagagac ttaaaacctc aaaatctctt
      721 gattgatgac aaaggaacaa ttaaactggc tgattttggc cttgccagag cttttggaat
      781 acctatcaga gtatatacac atgaggtagt aacactctgg tacagatctc cagaagtatt
      841 gctggggtca gctcgttact caactccagt tgacatttgg agtataggca ccatatttgc
      901 tgaactagca actaagaaac cacttttcca tggggattca gaaattgatc aactcttcag
      961 gattttcaga gctttgggca ctcccaataa tgaagtgtgg ccagaagtgg aatctttaca
     1021 ggactataag aatacatttc ccaaatggaa a
//
