LOCUS       sequence-103907-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cell cycle marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  TITLE     Pines Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pIRES-mRFP-NLS-PCNA (2514)
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           107..146
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             182..202
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             218..892
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             218..892
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(348..366)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     616..635
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     810..833
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgcct
       61 gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc ttggtggcgt
      121 gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtatggctt cgtggggatc
      181 cccgaagaag aagcgcaaag tactggtacc ggtcgacatg gcctcctccg aggacgtcat
      241 caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc acgagttcga
      301 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      361 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tccagtacgg
      421 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      481 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      541 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
      601 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
      661 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagatgaggc tgaagctgaa
      721 ggacggcggc cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca
      781 gctgcccggc gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta
      841 caccatcgtg gaacagtacg agcgcgccga gggccgccac tccaccggcg ccggcctgta
      901 caagg
//
