LOCUS       sequence-103908-        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for making stable RPE cells expressing WT cdc20.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  TITLE     Pines Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA5/FRT/TO(Neo)-Venus-Flag-WT wobbled cdc20...
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    39..57
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     67..91
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(251..253,254..964)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(293..314)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(554..573)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     901..922
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg
       61 tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      121 cctccataga agacaccggg accgatccag cctccggact ctagcgttta aacttaagat
      181 ggactacaaa gaccatgacg gtgattataa agatcatgac atcgattaca aggatgacga
      241 tgacggtacc gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      301 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      361 cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg
      421 gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct accccgacca
      481 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      541 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      601 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      661 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca
      721 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca
      781 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      841 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
      901 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
      961 caagggatcc atggcccaat ttgcctttga atccgatctt catagcctct tgcaactcga
     1021 cgcccctatt cctaacgccc ctcccgccag gtggcaaagg aaggctaaga ggct
//
