LOCUS       sequence-103911-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of cyclin B1 L45 A mutant fused to Venus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Matsusaka T, Enquist-Newman M, Morgan DO, Pines J
  TITLE     Co-activator independent differences in how the metaphase and 
            anaphase APC/C recognise the same substrate.
  JOURNAL   Biol Open. 2014 Sep 12;3(10):904-12. doi: 10.1242/bio.20149415.
  PUBMED    25217616
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/bio"; 
            journalName: "Biol Open"; date: "2014-09-12- 12"; volume: "3"; 
            issue: "10"; pages: "904-12"
COMMENT     Sequence Label: pVenus-N1 cyclin B1 L45A (1324)
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(146..859,860..862,863..865))
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(191..212)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     540..559
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     799..820
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      862..871
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatct agagtcgcgg ccgctttact tgtacagctc gtccatgccg agagtgatcc
      181 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
      241 tcagggcgga ctggtagctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
      301 tgggggtgtt ctgctggtag tggtcggcga gctgcacgcc gccgtcctcg atgttgtggc
      361 ggatcttgaa gttggccttg atgccgttct tctgcttgtc ggcggtgata tagacgttgt
      421 ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
      481 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
      541 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
      601 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg aagcactgta
      661 ggccgtagcc cagagtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
      721 agatcagctt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
      781 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
      841 acagctcctc gcccttgctc accatggtgg cgaccggtgg atcctctaga acctttgcca
      901 cagccttggc taaatcttga actagtgcag aattcagctg tggtagagtg ctgatcttag
      961 catgcttcga tgtggcatac ttgttcttga cagtcatgtg ctttgtaagt ccttgattta
     1021 ccatgactac attcttagcc aggtgctgca taactggaag a
//
