LOCUS       sequence-103912-        1112 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sucrose counterselection (sacB) vector for unmarked allelic 
            exchange..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Puri AW, Owen S, Chu F, Chavkin T, Beck DA, Kalyuzhnaya MG, Lidstrom
            ME
  TITLE     Genetic tools for the industrially promising methanotroph 
            Methylomicrobium buryatense.
  JOURNAL   Appl Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14.
  PUBMED    25548049
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14."
COMMENT     Sequence Label: pCM433kanT
FEATURES             Location/Qualifiers
     source          1..1112
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(571..942)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            975..1084
                     /note="incP origin of transfer"
ORIGIN
        1 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
       61 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
      121 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
      181 ttaccaatgt gcaggttgtc ggtgtcgtga tgcacggcac tgacgcgctg atgctcggcg
      241 aagccaagcc cagcgcagat gcggtcctca atcgcgcgca acgtctccgc gtcgggcttc
      301 tctcccgcgc ggaagctaac cagcaggtga taggtcttgt cggcctcgga acgggtgttg
      361 ccgtgctggg tcgccatcac ctcggccatg acagcgggca gggtgtttgc ctcgcagttc
      421 gtgacgcgca cgtgacccag gcgctcggtc ttgccttgct cgtcggtgat gtacttcacc
      481 agctccgcga agtcgctctt cttgatggag cgcatgggga cgtgcttggc aatcacgcgc
      541 accccccggc cgttttagcg gctaaaaaag tcatggctct gccctcgggc ggaccacgcc
      601 catcatgacc ttgccaagct cgtcctgctt ctcttcgatc ttcgccagca gggcgaggat
      661 cgtggcatca ccgaaccgcg ccgtgcgcgg gtcgtcggtg agccagagtt tcagcaggcc
      721 gcccaggcgg cccaggtcgc cattgatgcg ggccagctcg cggacgtgct catagtccac
      781 gacgcccgtg attttgtagc cctggccgac ggccagcagg taggccgaca ggctcatgcc
      841 ggccgccgcc gccttttcct caatcgctct tcgttcgtct ggaaggcagt acaccttgat
      901 aggtgggctg cccttcctgg ttggcttggt ttcatcagcc atccgcttgc cctcatctgt
      961 tacgccggcg gtagccggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg
     1021 cgaataaggg acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct
     1081 gcccggctga cgccgttgga tacaccaagg aa
//
