LOCUS       sequence-103920-        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A directional cloning vector of PCR-amplified cDNAs for mammalian 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  TITLE     Directional cloning vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUC-dirTopo-P2A-aGFP
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(59..163)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    213..334
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(224..243)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     278..297
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(506..527)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     855..874
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
       61 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
      121 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
      181 tgccacctga ttaacgctta caatttacgc gttaagatac attgatgagt ttggacaaac
      241 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
      301 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
      361 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
      421 tggtatggct gattatgatc agttatctag attactcgag ttacttgtac agctcgtcca
      481 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
      541 cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca
      601 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
      661 cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca
      721 tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt
      781 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
      841 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
      901 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
      961 ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca
     1021 gcttgccggt ggtgcaga
//
