LOCUS       sequence-103924-         581 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Zetsche B, Volz SE, Zhang F
  TITLE     A split-Cas9 architecture for inducible genome editing and 
            transcription modulation.
  JOURNAL   Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
  PUBMED    25643054
REFERENCE   2  (bases 1 to 581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149."
COMMENT     Sequence Label: PX852
FEATURES             Location/Qualifiers
     source          1..581
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             57..77
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             102..422
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 ctggagcacc tgcctgaaat cacttttttt caggttggac cggtgccacc atggccccaa
       61 agaagaagcg gaaggtcggt atccacggag tcccagcagc cggcgtgcag gtggagacca
      121 tcagccccgg cgacggcaga accttcccca agagaggcca gacctgcgtg gtgcactaca
      181 ccggcatgct ggaggacggc aagaagttcg acagcagcag agacagaaac aagcccttca
      241 agttcatgct gggcaagcag gaggtgatca gaggctggga ggagggcgtg gcccagatga
      301 gcgtgggcca gagagccaag ctgaccatca gccccgacta cgcctacggc gccaccggcc
      361 accccggcat catccctcct cacgccaccc tggtgttcga cgtggagctg ctgaagctgg
      421 agggtggcgg tggctcgggt ggcggtggct cgggtggcgg tggctcgaag cccgccttcc
      481 tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga
      541 ccgtgaagca gctgaaagag gactacttca agaaaatcga g
//
