LOCUS       sequence-103925-        2137 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Zetsche B, Volz SE, Zhang F
  TITLE     A split-Cas9 architecture for inducible genome editing and 
            transcription modulation.
  JOURNAL   Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
  PUBMED    25643054
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149."
COMMENT     Sequence Label: PX853
FEATURES             Location/Qualifiers
     source          1..2137
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..1793
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             1839..2108
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
ORIGIN
        1 ctggagcacc tgcctgaaat cacttttttt caggttggac cggtgccacc atgctagatt
       61 tagctagctt aatcctcgac aagaagtaca gcatcggcct ggacatcggc accaactctg
      121 tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg
      181 gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg
      241 gcgaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga
      301 agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca
      361 gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc
      421 accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct
      481 accacctgag aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc
      541 tggccctggc ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc
      601 ccgacaacag cgacgtggac aagctgttca tccagctggt gcagacctac aaccagctgt
      661 tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa ggccatcctg tctgccagac
      721 tgagcaagag cagacggctg gaaaatctga tcgcccagct gcccggcgag aagaagaatg
      781 gcctgttcgg aaacctgatt gccctgagcc tgggcctgac ccccaacttc aagagcaact
      841 tcgacctggc cgaggatgcc aaactgcagc tgagcaagga cacctacgac gacgacctgg
      901 acaacctgct ggcccagatc ggcgaccagt acgccgacct gtttctggcc gccaagaacc
      961 tgtccgacgc catcctgctg agcgacatcc tgagagtgaa caccgagatc accaaggccc
     1021 ccctgagcgc ctctatgatc aagagatacg acgagcacca ccaggacctg accctgctga
     1081 aagctctcgt gcggcagcag ctgcctgaga agtacaaaga gattttcttc gaccagagca
     1141 agaacggcta cgccggctac attgacggcg gagccagcca ggaagagttc tacaagttca
     1201 tcaagcccat cctggaaaag atggacggca ccgaggaact gctcgtgaag ctgaacagag
     1261 aggacctgct gcggaagcag cggaccttcg acaacggcag catcccccac cagatccacc
     1321 tgggagagct gcacgccatt ctgcggcggc aggaagattt ttacccattc ctgaaggaca
     1381 accgggaaaa gatcgagaag atcctgacct tccgcatccc ctactacgtg ggccctctgg
     1441 ccaggggaaa cagcagattc gcctggatga ccagaaagag cgaggaaacc atcaccccct
     1501 ggaacttcga ggaagtggtg gacaagggcg cttccgccca gagcttcatc gagcggatga
     1561 ccaacttcga taagaacctg cccaacgaga aggtgctgcc caagcacagc ctgctgtacg
     1621 agtacttcac cgtgtataac gagctgacca aagtgaaata cgtgaccgag ggaatgagaa
     1681 agcccgcctt cctgagcggc gagcagaaaa aggccatcgt ggacctgctg ttcaagacca
     1741 accggaaagt gaccgtgaag cagctgaaag aggactactt caagaaaatc gagggtggcg
     1801 gtggctcggg tggcggtggc tcgggtggcg gtggctcgga gatgtggcac gagggcctgg
     1861 aggaggccag cagactgtac ttcggcgaga gaaacgtgaa gggcatgttc gaggtgctgg
     1921 agcccctgca cgccatgatg gagagaggcc cccagaccct gaaggagacc agcttcaacc
     1981 aggcctacgg cagagacctg atggaggccc aggagtggtg cagaaagtac atgaagagcg
     2041 gcaacgtgaa ggacctgacc caggcctggg acctgtacta ccacgtgttc agaagaatca
     2101 gcaagcagta agaattccta gagctcgctg atcagcc
//
