LOCUS       sequence-103936-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TvoVMAN11 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-110
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..59)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(127..417)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             complement(424..441)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    497..521
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(521..540)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(522..540)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        586..663
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(683..702)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gctgcgctag tagacgagtc catgtgctgg cgttcaaatt tcgcagcagc ggtttcttta
       61 ccagactcga gtgcggccgc aagcttacgc aaggtaaact ggtgtttcac ctgatacgca
      121 cttggatcca ccaatctgtt ctctgtgagt ctcaataata tcgttatcct ccatgtccaa
      181 atcttcaggg gtctgatcag cttgaattct aataccgtcg tacaagaatc ttaaggagtc
      241 catttcctta ccctgtcttt tagcgaacgc ttccatcagc cttcttaaag gagtggtctt
      301 tttgatctta aagaagattt ctgaagatcc atcggacacc tttaaattga tgtgagtctc
      361 aggcttgact tctggcttga cctctggctt agcttcttga ttgacttctg agtcactagt
      421 gctgtgatga tgatgatgat ggctgctgcc catggtatat ctccttatta aagttaaaca
      481 aaattatttc tacaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta
      541 atttcctaat gcaggagtcg cataagggag agcgtcgaga tcccggacac catcgaatgg
      601 cgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg
      661 aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc
      721 gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa
      781 gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa
//
