LOCUS       sequence-103938-        3193 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-term SpCas9 piece of inducible transcriptional activator 
            (dCas9(C)-FKBP-2xNLS-VP64).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Zetsche B, Volz SE, Zhang F
  TITLE     A split-Cas9 architecture for inducible genome editing and 
            transcription modulation.
  JOURNAL   Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
  PUBMED    25643054
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149."
COMMENT     Sequence Label: PX856
FEATURES             Location/Qualifiers
     source          1..3193
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             57..77
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             102..422
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             join(468..1448,1449..1451,1452..2966)
                     /codon_start=1
                     /product="C-terminal portion of catalytically inactive 
                     Streptococcus pyogenes dCas9 carrying the N863A mutation 
                     (Zetsche et al., 2015)"
                     /label=dCas9(C)
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     CDS             2970..2990
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3009..3158
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 ctggagcacc tgcctgaaat cacttttttt caggttggac cggtgccacc atggccccaa
       61 agaagaagcg gaaggtcggt atccacggag tcccagcagc cggcgtgcag gtggagacca
      121 tcagccccgg cgacggcaga accttcccca agagaggcca gacctgcgtg gtgcactaca
      181 ccggcatgct ggaggacggc aagaagttcg acagcagcag agacagaaac aagcccttca
      241 agttcatgct gggcaagcag gaggtgatca gaggctggga ggagggcgtg gcccagatga
      301 gcgtgggcca gagagccaag ctgaccatca gccccgacta cgcctacggc gccaccggcc
      361 accccggcat catccctcct cacgccaccc tggtgttcga cgtggagctg ctgaagctgg
      421 agggtggcgg tggctcgggt ggcggtggct cgggtggcgg tggctcgaag cccgccttcc
      481 tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga
      541 ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa
      601 tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa
      661 ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg
      721 tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg
      781 cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg
      841 gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc
      901 tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg
      961 acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc
     1021 tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga
     1081 cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg
     1141 tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga
     1201 gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc
     1261 ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc
     1321 gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacc
     1381 atatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa
     1441 gcgacaaggc ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga
     1501 agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc
     1561 tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac
     1621 agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga
     1681 acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt
     1741 ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca
     1801 actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa
     1861 agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga
     1921 agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca
     1981 gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc
     2041 ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt
     2101 ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg
     2161 tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga
     2221 tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg
     2281 cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg
     2341 tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg
     2401 actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta
     2461 agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgaac
     2521 tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca
     2581 gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg
     2641 aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag
     2701 tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata
     2761 agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag
     2821 cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca
     2881 aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga
     2941 tcgacctgtc tcagctggga ggcgacagcc ccaagaagaa gagaaaggtg gaggccagcg
     3001 gacgggctga cgcattggac gattttgatc tggatatgct gggaagtgac gccctcgatg
     3061 attttgacct tgacatgctt ggttcggatg cccttgatga ctttgacctc gacatgctcg
     3121 gcagtgacgc ccttgatgat ttcgacctgg acatgctgat taactaagaa ttcctagagc
     3181 tcgctgatca gcc
//
