LOCUS       sequence-103939-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TvoVMAdC48 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-343
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..366
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             815..859
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 tgtaaataat tttgtttaac tttaataagg agatatacca tgggcagcag ccatcatcat
       61 catcatcaca gcactagtga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca
      121 gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agaaatcttc
      181 tttaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag
      241 ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag
      301 acccctgaag atttggacat ggaggataac gatattattg agactcacag agaacagatt
      361 ggtggatcca agtgcgtatc aggtgaaaca ccagtttacc ttgcggatgg caagacaata
      421 aaaataaagg atctatacag ttctgagaga aaaaaagaag ataacattgt tgaggctggt
      481 tcgggagaag agataataca tctaaaagat cccattcaaa tatattctta tgtggacggg
      541 accatagtca ggagcagatc aagacttcta tacaagggca agagctctta tcttgtgagg
      601 atagaaacta ttggcggaag atcggtaagc gttacaccag ttcacaaact ctttgtcctt
      661 acggaaaagg gtatcgaaga ggttatggcc tccaacctaa aggtaggcga catgattgct
      721 gctgtagcag aaagcgaatc tgaagcaaga gactgcggaa tgagcgagga atgcgtgatg
      781 gaagcataag cttgcggccg cactcgagtc tggtaaagaa accgctgctg cgaaatttga
      841 acgccagcac atggactcgt ctactagcgc agcttaat
//
