LOCUS       sequence-103940-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TvoVMAdC48 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-343
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..60)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(509..799)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             complement(806..823)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agctgcgcta gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt
       61 accagactcg agtgcggccg caagcttatg cttccatcac gcattcctcg ctcattccgc
      121 agtctcttgc ttcagattcg ctttctgcta cagcagcaat catgtcgcct acctttaggt
      181 tggaggccat aacctcttcg ataccctttt ccgtaaggac aaagagtttg tgaactggtg
      241 taacgcttac cgatcttccg ccaatagttt ctatcctcac aagataagag ctcttgccct
      301 tgtatagaag tcttgatctg ctcctgacta tggtcccgtc cacataagaa tatatttgaa
      361 tgggatcttt tagatgtatt atctcttctc ccgaaccagc ctcaacaatg ttatcttctt
      421 tttttctctc agaactgtat agatccttta tttttattgt cttgccatcc gcaaggtaaa
      481 ctggtgtttc acctgatacg cacttggatc caccaatctg ttctctgtga gtctcaataa
      541 tatcgttatc ctccatgtcc aaatcttcag gggtctgatc agcttgaatt ctaataccgt
      601 cgtacaagaa tcttaaggag tccatttcct taccctgtct tttagcgaac gcttccatca
      661 gccttcttaa aggagtggtc tttttgatct taaagaagat ttctgaagat ccatcggaca
      721 cctttaaatt gatgtgagtc tcaggcttga cttctggctt gacctctggc ttagcttctt
      781 gattgacttc tgagtcacta gtgctgtgat gatgatgatg atggctgctg cccatggtat
      841 atctccttat taaagttaaa caaaattatt tctacagg
//
