LOCUS       sequence-103944-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PhoRadAdC46 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-344
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..357
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             773..817
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 ttttgtttaa ctttaataag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcactagtg actcagaagt caatcaagaa gctaagccag aggtcaagcc agaagtcaag
      121 cctgagactc acatcaattt aaaggtgtcc gatggatctt cagaaatctt ctttaagatc
      181 aaaaagacca ctcctttaag aaggctgatg gaagcgttcg ctaaaagaca gggtaaggaa
      241 atggactcct taagattctt gtacgacggt attagaattc aagctgatca gacccctgaa
      301 gatttggaca tggaggataa cgatattatt gagactcaca gagaacagat tggtggatcc
      361 gggaagtgct ttgctaggga taccgaagtt tattatgaaa acgatactgt accacacatg
      421 gaatcaattg aggagatgta tagtaaatac gcctcgatga atggggaatt accattcgat
      481 aatggttacg cagttccatt agataatgtc tttgtgtaca cgttggacat cgctagtggt
      541 gaaattaaga aaacgagggc ttcatacatc tatcgcgaga aggttgagaa gctcattgag
      601 ataaaactat ccagtggata ctcccttaag gttactccct ctcacccagt tctcctcttt
      661 agagatggcc tgcaatgggt ccccgctgct gaagttaaac ctggagatgt tgttgttggt
      721 gttagggaag aggtactgag aagataagct tgcggccgca ctcgagtctg gtaaagaaac
      781 cgctgctgcg aaatttgaac gccagcacat ggactcgtct actagcgcag cttaattaac
      841 ctaggctgct gccaccgctg agcaataact a
//
