LOCUS       sequence-103947-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PhoRadAdC46 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-344
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..60)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(476..766)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             complement(773..790)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agctgcgcta gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt
       61 accagactcg agtgcggccg caagcttatc ttctcagtac ctcttcccta acaccaacaa
      121 caacatctcc aggtttaact tcagcagcgg ggacccattg caggccatct ctaaagagga
      181 gaactgggtg agagggagta accttaaggg agtatccact ggatagtttt atctcaatga
      241 gcttctcaac cttctcgcga tagatgtatg aagccctcgt tttcttaatt tcaccactag
      301 cgatgtccaa cgtgtacaca aagacattat ctaatggaac tgcgtaacca ttatcgaatg
      361 gtaattcccc attcatcgag gcgtatttac tatacatctc ctcaattgat tccatgtgtg
      421 gtacagtatc gttttcataa taaacttcgg tatccctagc aaagcacttc ccggatccac
      481 caatctgttc tctgtgagtc tcaataatat cgttatcctc catgtccaaa tcttcagggg
      541 tctgatcagc ttgaattcta ataccgtcgt acaagaatct taaggagtcc atttccttac
      601 cctgtctttt agcgaacgct tccatcagcc ttcttaaagg agtggtcttt ttgatcttaa
      661 agaagatttc tgaagatcca tcggacacct ttaaattgat gtgagtctca ggcttgactt
      721 ctggcttgac ctctggctta gcttcttgat tgacttctga gtcactagtg ctgtgatgat
      781 gatgatgatg gctgctgccc atggtatatc tccttattaa agttaaacaa aattatttct
      841 acagggga
//
