LOCUS       sequence-103949-         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing 
            mCherry. Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR mCherry L5-L2
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..78
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(298..316)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     566..585
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
       61 ctttgtatac aaaagttggg agacccaagc ttggtaccga gctcggatcc ggaggctttt
      121 ttggaggcct aggcttttgc aaaaagctta ccatggtgag caagggcgag gaggataaca
      181 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
      241 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      301 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
      361 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
      421 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
      481 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
      541 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
      601 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
      661 tgaagctg
//
