LOCUS       sequence-103950-         673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing 
            mCherry. Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR mCherry L5-L2
FEATURES             Location/Qualifiers
     source          1..673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..113
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(423..442)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgcagctgga tggcaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
       61 aattgataag caatgctttc ttataatgcc aactttgtac aagaaagctg ggtacctcta
      121 gatgcatgct cgaggattgt cgacttactt gtacagctcg tccatgccgc cggtggagtg
      181 gcggccctcg gcgcgttcgt actgttccac gatggtgtag tcctcgttgt gggaggtgat
      241 gtccaacttg atgttgacgt tgtaggcgcc gggcagctgc acgggcttct tggccttgta
      301 ggtggtcttg acctcagcgt cgtagtggcc gccgtccttc agcttcagcc tctgcttgat
      361 ctcgcccttc agggcgccgt cctcggggta catccgctcg gaggaggcct cccagcccat
      421 ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg gtgccgcgca gcttcacctt
      481 gtagatgaac tcgccgtcct gcagggagga gtcctgggtc acggtcacca cgccgccgtc
      541 ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggacagct tcaagtagtc
      601 ggggatgtcg gcggggtgct tcacgtaggc cttggagccg tacatgaact gaggggacag
      661 gatgtcccag gcg
//
