LOCUS       sequence-103954-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Oeemig JS, Aranko AS, Djupsjobacka J, Heinamaki K, Iwai H
  TITLE     Solution structure of DnaE intein from Nostoc punctiforme: 
            structural basis for the design of a new split intein suitable for 
            site-specific chemical modification.
  JOURNAL   FEBS Lett. 2009 May 6;583(9):1451-6. doi: 
            10.1016/j.febslet.2009.03.058. Epub 2009 Apr 1.
  PUBMED    19344715
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.febslet.2009.03.058"; journalName: "FEBS Lett"; date: 
            "2009-05-6- 6"; volume: "583"; issue: "9"; pages: "1451-6"
COMMENT     Sequence Label: pHYDuet93
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttaa ctttaataag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccta ggacagtaca aacttatcct gaacggtaaa
      121 accctgaaag gtgaaaccac caccgaagct gttgacgctg ctaccgcgga aaaagttttc
      181 aaacagtacg ctaacgacaa cggtgttgac ggtgaatgga cctacgacga cgctaccaaa
      241 acctacaccg taacggaagg atcctgttta agctatgaaa cggaaatatt gacagtagaa
      301 tatggattat taccgattgg taaaattgta gaaaagcgca tcgaatgtac tgtttatagc
      361 gttgataata atggaaatat ttatacacaa cctgtagcac aatggcacga tcgcggagaa
      421 caagaggtgt ttgagtattg tttggaagat ggttcattga ttcgggcaac aaaagaccat
      481 aagtttatga ctgttgatgg tcaaatgttg ccaattgatg aaatatttga acgtgaattg
      541 gatttgatgc gggttgataa tttgccgaat atcaaaatag ccacacgtaa atatttaggc
      601 aaacaaaatg tctatgacat tggagttgag cgcgaccata attttgcact caaaaattaa
      661 gctttagctt ctaattgttt caatggtacc cagtacaaac ttatcctgaa cggtaaaacc
      721 ctgaaaggtg aaaccaccac cgaagctgtt gacgctgcta ccgcggaaaa agttttcaaa
      781 cagtacgcta acgacaacgg tgttgacggt gaatggacct acgacgacgc taccaaaacc
      841 tacaccgtaa cggaataagc tt
//
