LOCUS       sequence-103956-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Oeemig JS, Aranko AS, Djupsjobacka J, Heinamaki K, Iwai H
  TITLE     Solution structure of DnaE intein from Nostoc punctiforme: 
            structural basis for the design of a new split intein suitable for 
            site-specific chemical modification.
  JOURNAL   FEBS Lett. 2009 May 6;583(9):1451-6. doi: 
            10.1016/j.febslet.2009.03.058. Epub 2009 Apr 1.
  PUBMED    19344715
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.febslet.2009.03.058"; journalName: "FEBS Lett"; date: 
            "2009-05-6- 6"; volume: "583"; issue: "9"; pages: "1451-6"
COMMENT     Sequence Label: pHYDuet93
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..60)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(862..879)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(889..906)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agctgcgcta gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt
       61 accagactcg agccgccaaa acagccaagc ttattccgtt acggtgtagg ttttggtagc
      121 gtcgtcgtag gtccattcac cgtcaacacc gttgtcgtta gcgtactgtt tgaaaacttt
      181 ttccgcggta gcagcgtcaa cagcttcggt ggtggtttca cctttcaggg ttttaccgtt
      241 caggataagt ttgtactggg taccattgaa acaattagaa gctaaagctt aatttttgag
      301 tgcaaaatta tggtcgcgct caactccaat gtcatagaca ttttgtttgc ctaaatattt
      361 acgtgtggct attttgatat tcggcaaatt atcaacccgc atcaaatcca attcacgttc
      421 aaatatttca tcaattggca acatttgacc atcaacagtc ataaacttat ggtcttttgt
      481 tgcccgaatc aatgaaccat cttccaaaca atactcaaac acctcttgtt ctccgcgatc
      541 gtgccattgt gctacaggtt gtgtataaat atttccatta ttatcaacgc tataaacagt
      601 acattcgatg cgcttttcta caattttacc aatcggtaat aatccatatt ctactgtcaa
      661 tatttccgtt tcatagctta aacaggatcc ttccgttacg gtgtaggttt tggtagcgtc
      721 gtcgtaggtc cattcaccgt caacaccgtt gtcgttagcg tactgtttga aaactttttc
      781 cgcggtagca gcgtcaacag cttcggtggt ggtttcacct ttcagggttt taccgttcag
      841 gataagtttg tactgtccta ggctgccgcg cggcaccagg ccgctgctgt gatgatgatg
      901 atgatggctg ctgcccatgg tatatct
//
